Extended Data Fig. 6: Increased survival in phenotypic obese and mice that were formerly obese with 8-week HFD exposure.
From: Diet switch pre-vaccination improves immune response and metabolic status in formerly obese mice
(a) Timeline of diet administration, vaccination, and challenge in half-term diet switch studies. (b) Weights of mice through a half-term diet switch studies (n = 5–10 mice/group) at (c) vaccination and challenge (vaccination always lean p < 0.0001; H1N1 always lean p < 0.0001, formerly obese p < 0.0001). (d–f) Morbidity and mortality upon H1N1 virus challenge with n = 5–10 mice/group. (d) Weight curves with grey dotted line indicate a 0% deviation from baseline weight, (e) clinical scores and (f) survival. (g) Hemagglutination inhibition titers at indicated timepoints. The grey dotted line indicates the lower limit of detection. Data are represented in (b-e) as means ± standard error, in (f) as surviving proportions with censored or event animals indicated by their respective symbols, and in (g) as geometric mean ± standard deviation with x indicating no mice surviving to time point. Statistical comparisons test between mice with obesity and indicated diet groups (n = 10 mice/group) in (c).
