Extended Data Fig. 6: P. anaerobius produced metabolites to modulate MDSCs, further influencing T cell function. | Nature Microbiology

Extended Data Fig. 6: P. anaerobius produced metabolites to modulate MDSCs, further influencing T cell function.

From: Peptostreptococcus anaerobius mediates anti-PD1 therapy resistance and exacerbates colorectal cancer via myeloid-derived suppressor cells in mice

Extended Data Fig. 6

a. Schematic of experiments design. MDSCs were treated with 10% culture medium from Caco-2 pre-incubated with P. anaerobius for 24 h. mRNA expressions level of Arg1 and iNOS were detected using RT-qPCR (n = 3 biologically independent samples). b. Schematic diagram of experimental design. MDSCs treated with 10% P. anaerobius-conditioned medium (Pa-CM) or E. coli-conditioned medium (Ec-CM) were harvested after 24 h treatment. c. Primary T cells were treated with 10% Pa. CM for 24 h. The percentage of functional CD4+ and CD8+ T cell was detected using FACS (n = 6-10 biologically independent samples). d. Different molecular fractions from Pa-CM were used to treat MDSCs. e. MDSCs were treated with 50-100 kDa fraction from Pa-CM or control broth. Data are presented as mean ± SEM. P values were calculated unpaired two-tailed Student’s t-test.

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