Extended Data Fig. 1: P. anaerobius promotes an immune suppressive tumour microenvironment in CRC progression.
a. Absolute number of CD4+T cells and CD8+T cells in colonic lamina propria (LP) cells from Apc min/+ mice. PBS (n = 12), E.coli (n = 9) and P. anaerobius (n = 10). b. Representative immunofluorescent staining picture and quantification of CD11b+Gr-1+cells in colon from Apc min/+ mice. Scale bar: 25μm. c. Experimental design for P. anaerobius treatment in AOM-induced CRC mouse model. Representative images of mouse colonoscopy at week 33 (right). d. Representative images of colon at sacrifice (left). Tumour number and volume. PBS (n = 8), P. anaerobius (n = 11). e. Absolute number of MDSCs, (f) IFNγ+CD8+T cells and IFNγ+CD4+T cells in colonic LP. 8 mice were used in each group, including PBS (n = 8), P. anaerobius (n = 8). g. Absolute number of CD4+T cells and CD8+T cells in colonic LP cells. PBS (n = 7) and P. anaerobius (n = 11). h-i. Representative immunofluorescent staining picture and quantification of CD11b+Gr-1+cells in tumour (h) and colon (i). Scale bar: 25 μm. j. Experimental design for P. anaerobius treatment in AOM/DSS-induced CRC mouse model (Right). Representative images of colon at sacrifice (middle). Tumour burden(right). PBS (n = 11), E.coli (n = 12) and P. anaerobius (n = 10). k. Percentage of MDSCs, (l) IFNγ+CD8+T cells, and (m) Innate lymphoid cells (ILCs), plasmacytoid dendritic cells (pDCs), myeloid dendritic cells (mDCs), γδ T cells in the colonic lamina propria (LP). k-m PBS (n = 8), E.coli (n = 9) and P. anaerobius (n = 8). Data are presented as mean ± SEM. P values were calculated by one-way ANOVA followed by Tukey’s post-hoc test (a-b, j-l) and unpaired two-tailed Student’s t-test (d-i).
