Extended Data Fig. 6: Fluorescence microscopy to probe oligomerization of lipid II binding drugs. | Nature Microbiology

Extended Data Fig. 6: Fluorescence microscopy to probe oligomerization of lipid II binding drugs.

From: Host defence peptide plectasin targets bacterial cell wall precursor lipid II by a calcium-sensitive supramolecular mechanism

Extended Data Fig. 6

GUVs consisting of 0.4 mol% Und-NBD-LII in DOPC were imaged after addition of the antibiotic. A representative blank is shown for untreated GUVs. A) Schematic structure of the lipid II dye used in this work with the tag at the end of the C55-isoprenyl tail (Und-NBD-LII) and B) of the previously reported lipid II dye with the NBD-tag attached to the sidechain of lysine-3 of lipid II (K3-NBD-LII) C) Blank D) 1 μM plectasin E) 1 μM copsin, a CSαβ-defensin also reported to bind to lipid II F) 1 μM nisin, a lantibiotic well-known to form supramolecular pores upon lipid II binding. G) 1 μM teixobactin, a depsi-peptide that targets lipid II and subsequently forms fibrils on top of the membrane. H) 1 μM ramoplanin, a glycolipodepsipeptide used in the clinic that binds lipid II. I) 1 μM teicoplanin, a glycopeptide also used in the clinic that targets lipid II.

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