Extended Data Fig. 9: ssNMR of plectasin in bacterial membrane preparations.
Comparison of ssNMR amide fingerprints of plectasin bound to Lipid II in bacterial membrane preparations and model membranes. Spectra of plectasin in bacterial membrane preparations were recorded at a magnetic field of 16.4 T (700 MHz 1H frequency), 60 kHz MAS and a sample temperature of 305 K. Spectra of plectasin in model membranes were recorded at 28.2 T (1200 MHz 1H frequency) 60 kHz MAS and a sample temperature of 305 K, with the exception of DOPG without calcium, which was recorded at 16.4 T (700 MHz 1H frequency). A) Overlay of an NH spectrum acquired in M. flavus membranes (blue) with an NH spectrum acquired in DOPC without added calcium (green) B) Same as A) but with spectrum acquired in S. simulans membranes (yellow). In S. simulans the spectrum is highly heterogeneous, while in M. flavus it is much more defined. Large apparent 15N chemical shift perturbations of the histidine sidechains in A and B are due to differences in spectral width of the indirectly detected dimension. C), D), E), F) & G) Calculated CSPs of plectasin in M. flavus membranes compared to in DOPC (C), DOPC with Ca2+ (D), DOPG (E), DOPG with Ca2+ (F), and DOPC with Mg2+ (G). Source data are provided for C-G).
