Extended Data Fig. 6: Longitudinal analysis and overall comparison of Active MS SLCLs (AMS) with Stable SLCLs (SMS). | Nature Microbiology

Extended Data Fig. 6: Longitudinal analysis and overall comparison of Active MS SLCLs (AMS) with Stable SLCLs (SMS).

From: Multiple sclerosis patient-derived spontaneous B cells have distinct EBV and host gene expression profiles in active disease

Extended Data Fig. 6

(a) Comparison of viral DNA load by ddPCR and (b) EBV LF3 transcript levels by RT-qPCR for SMS2 vs AMS5 (Patient A) and SMS5 vs AS2 (Patient B) (two patients, two timepoints per patient). (c) Heat map comparing all AMS vs SMS showing top 930 differentially regulated genes with p < .05. The gene expression values were log2 normalized and mean-centered to highlight relative changes (SMS n = 6; AMS n = 5). (d) Gene expression (normalized counts) for all AMS (red) or SMS (green) SLCLs for light chain/immunoglobulin κ, MHC class II DP, MHC class II DR, and IL-6 (SMS n = 6; AMS n = 5, DESeq Wald test). All data points represent distinct samples tested from paired-end RNA-seq data set. Data for (d) are mean ± SD.

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