Extended Data Fig. 7: FOXP1 knockdown downregulates EBV lytic gene expression in AMS SLCLs and Mutu-I Burkitt’s lymphoma cells. | Nature Microbiology

Extended Data Fig. 7: FOXP1 knockdown downregulates EBV lytic gene expression in AMS SLCLs and Mutu-I Burkitt’s lymphoma cells.

From: Multiple sclerosis patient-derived spontaneous B cells have distinct EBV and host gene expression profiles in active disease

Extended Data Fig. 7

(a) RT-qPCR expression of FOXP1 expression relative to GUSB in AMS4 SLCLs treated with 3 individual shRNAs specific for FOXP1 and control shRNA (n = 3 per siRNA treatment). (b) Western blot showing of EBV latent (EBNA1 and LMP1) and lytic (EA-D and Zta) genes, and FOXP1 in shRNA control and FOXP1 shRNA treated cells (n = 3 per siRNA treatment, TTEST). (c) Western blot showing three biological replicates of shFOXP1 and shCtrl treated Mutu-I cells probed for expression of FOXP1 and EBV lytic gene Zta and EA-D (n = 3 per siRNA treatment, TTEST). (d) Zta expression (mean fluorescence intensity) in Mutu-I cells treated with shFOXP1 or shCtrl ((n = 3 per siRNA treatment, one-way ANOVA followed by Tukey’s multiple comparison test). All data points represent distinct samples tested in triplicate. Data are mean ± SD.

Source data

Back to article page