Extended Data Fig. 8: LTA knockdown decreases viability and increases EBV lytic gene expression in SLCLs. | Nature Microbiology

Extended Data Fig. 8: LTA knockdown decreases viability and increases EBV lytic gene expression in SLCLs.

From: Multiple sclerosis patient-derived spontaneous B cells have distinct EBV and host gene expression profiles in active disease

Extended Data Fig. 8

(a–c) AMS2 SLCLs were treated with 3 individual shRNAs specific for LTA or control shRNA (pLKO) and assayed for (a) fold change in LTA RNA expression by RT-qPCR, (b) expression of Zta or (c) EA-D by RT-qPCR, and (d) cell viability. (e–j) HC SLCLs (n = 2, triplicate samples per treatment for each cell line); SMS SLCLs (n = 2, triplicate samples per treatment for each cell line; and AMS SLCLs (n = 2); triplicate samples per treatment for each cell line) treated with shLTA1.1 or shCtrl and assayed for (e) LTA RNA expression by RT-qPCR, (f) LTA/TNFβ protein expression in cell supernatant by ELISA (g) Zta expression by RT-pPCR (h) EA-D expression by RT-qPCR, (i) EBV DNA copies per cell by ddPCR, and (j) cell viability by CellTitreGlo. BJAB used as negative control cell line. (one-way ANOVA followed by Tukey’s multiple comparison test). All data points represent distinct samples tested in triplicate. Data are mean ± SD.

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