Extended Data Fig. 5: K. oxytoca toxins alone inhibit S. Tm partially, but complete inhibitory phenotype requires toxins and metabolic active bacteria.
(a) OD600 of S. Tm in LB supplemented with various concentrations of TM (0 µM to 320 µM), (b) TV (0 µM to 32 µM) and (c) EtOH as a solvent control (0 µM to 320 µM) over 24 h. (a-c) The mean ±SEM of one out of two biological experiment with n = 2 technical replicates is displayed. (d) CFUs of various Salmonella enterica serovars in LB media supplemented with 320 µM TV or EtOH as solvent control. (d) The mean ± SEM of n = 2 independent biological experiments with n = 2-3 technical replicates per group are displayed. (E) K. oxytoca MK01 WT and sKO CFUs after co-cultivation with S. Tm in the absence or presence of 320 µM TM or 32 µM TV. (f) CI of S. Tm after 24 h of co-cultivation with K. oxytoca MK01 WT and sKO strains in TLB media supplemented with 320 µM TM or 32 µM TV. Solid line indicates starting ratio of bacteria (index = 0.1). (e, f) The mean ± SEM of n = 3 independent experiments with n = 2-3 technical replicates per group are displayed. (g) Representative scanning electron microscopy pictures at 4,000 X magnification of S. Tm in TLB media without and with 320 µM TM. Bar represents 2 µm. Average size of cells obtained from 10 microscopic images at randomly selected positions of S. Tm in TLB media without and with 320 µM TM after 4 h of aerobic cultivation. P-value indicated represents two-tailed Mann-Whitney U-test between groups with ****p < 0.0001.
