Extended Data Fig. 6: K.oxytoca toxin production plays a major role in disturbed microbiota settings with high amounts of free sugars.
(a) CI of S. Tm after 24 h of co-cultivation with K. oxytoca MK01 WT, sKO and cWT strains in various semi-defined and defined culture media. (b) Corresponding S. Tm and (c) K. oxytoca CFUs after co-cultivation in various semi-defined and defined culture media. (a-c) The mean ± SEM of n = 3 independent experiments with n = 2-technical replicates per group are displayed. (d) S. Tm was co-cultured with three different K. oxytoca WT and tKO mutant strains (MK01, LK03 and MR06) in TLB with various concentrations of lactose. Resulting S. Tm CFUs are displayed. The mean ± SEM of n = 2 independent experiments with n = 2-technical replicates per group are displayed. (e) Area under curve (AUC) for TM and TV in sample supernatants of different WT and tKO strains of K. oxytoca after 24 h of co-cultivation with S. Tm in TLB medium with 10 g/l lactose. The mean ± SEM of three independent cultures are displayed. (f) S. Tm CFUs after various time points (every 2 h over 24 h) of co-cultures with MK01 WT, sKO and cWT strains in TLB and (g) corresponding AUCs for TM and (h) TV (F-H) The mean ± SEM of n = 2 independent experiments with n = 2 technical replicates are displayed. (i) K. oxytoca CFUs (upper panel) and corresponding CI (lower panel) of co-cultures with S. Tm in tryptone broth with various mono-, di- and oligo-saccharides as carbon sources (10 g/L). Solid line indicates starting ratio of bacteria (index = 0.1). The mean ± SEM of n = 2-3 independent experiments performed in n = 3 technical replicates are displayed. (j) S. Tm and (k) K. oxytoca CFUs after co-cultivation in sterile filtered cecal content supernatants of GF, OMM12, SPF and antibiotic treated SPF mice (abx). Dashed lines display limit of detection. (j-k) The mean ± SEM of four independent experiments with n = 2-technical replicates per group are displayed.
