Fig. 2: Broad protective capability is shared among many K. oxytoca and K. grimontii but not K. michiganensis strains.
a, Aerobic co-cultures of different S. enterica serovars (Typhimurium, Enteritidis, Infantis, Kentucky, Choleraesuis, Agona and Derby) with K. oxytoca MK01/DSM5175T or E. coli Mt1b1 in a 1:10 ratio in isolated GF caecal content. b, Resulting CI of various S. enterica serovars against K. oxytoca MK01, DSM5175T and E. coli Mt1b1. The horizontal dashed line indicates the starting ratio of bacteria (index = 0.1). The mean ± s.e.m. of n = 2 biological experiments with n = 2 technical replicates are displayed. ST, sequence type. c, S. Typhimurium was co-cultivated with different KoSC strains from different origins as described before. d, Resulting CI of S. Typhimurium colony-forming units in co-cultures with various strains from the KoSC species, K. oxytoca, K. michiganensis and K. grimontii. The dashed line indicates the starting ratio of bacteria (index = 0.1). The different colours of each dot represent the origin of the KoSC isolate in each co-culture. Each dot represents the mean of n = 2–3 independent experiments with n = 2–3 technical replicates. e, Phylogenetic distribution of the 122 KoSC isolates in comparison with 75 publicly available genomes including three type strains depicted in the inner ring. The species identity of the strains (second inner ring), the phenotype against S. Typhimurium in the ex vivo co-culture screening (third ring) and their names are indicated. The tree is constructed based on the sequence variations within 712 core genes using fasttree. See also Extended Data Fig. 3.
