Extended Data Fig. 2: K. oxytoca MK01 inhibits S. Tm better than two reference bacteria.
Aerobic co-cultures of S. Tm with Enterobacteria (K. oxytoca MK01, K. oxytoca DSM5175T or E. coli Mt1b1) in in germfree cecal content with varying ratios and incubation times. Resulting CFUs of (a) S. Tm and (b) Enterobacteria in a 1:10 ratio and (c) corresponding competitive index (CI). Resulting CFUs of (d) S. Tm and (e) Enterobacteria in a 1:1 ratio and (f) corresponding CI. (g) Resulting CFUs of S. Tm and (h) Enterobacteria in a 10:1 ratio and (I) corresponding CI. (j-o) Titration of various inoculation densities in a 1:1 and 1:10 ratio for S. Tm. vs. K. oxytoca MK01, K. oxytoca DSM5175T and E. coli Mt1b1 after 24 h of aerobic cultivation. (j) Resulting CFUs of various inoculation densities of S. Tm in a 1:1 and (k) 1:10 starting ratio. (l) Resulting CFUs of various inoculation densities of the enterobacterial strains in a 1:1 and (m) 1:10 starting ratio. (n) Corresponding CI of both bacteria at various inoculation densities and a starting ratio of 1:1 or (o) 1:10. (a-n) The mean ± SEM of one experiment with n = 3 technical replicates is displayed.
