Extended Data Fig. 6: Roles of mGluR2 in H5N6 and H7N9 virus internalization.
From: Influenza virus uses mGluR2 as an endocytic receptor to enter cells
a and b, Replication of H5N6 (a) and H7N9 (b) was significantly impaired in mGluR2-silenced A549 cells. c and d, Internalization of H5N6 (c) and H7N9 (d) viruses in mGluR2-knockdown A549 cells was significantly lower than that for control cells. e, Interaction of H5 HA and mGluR2 demonstrated by co-immunoprecipitation with the anti-Flag antibody coupled agarose beads. f, Interaction of H7 HA and mGluR2 demonstrated by co-immunoprecipitation with the anti-Flag antibody coupled agarose beads. g, Soluble protein mGluR2-GST treatment significantly reduced H5N6 virus replication in A549 cells. h, Soluble protein mGluR2-GST treatment significantly reduced H7N9 virus replication in A549 cells. i, mGluR2-ma treatment significantly reduced H5N6 virus replication in A549 cells. j, mGluR2-ma treatment significantly reduced H7N9 virus replication in A549 cells. The images in i and j are representative of three independent experiments. Error bar in panels a–d and g–j indicates the standard deviation. The data shown in panels a–d and g–j are means ± s.d. (n = 3 biologically independent experiments). Statistical analysis was performed by using the unpaired, two-tailed Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values are available in Source Data.
