Extended Data Fig. 1: Result of the ion channel RNAi screen.
From: Influenza virus uses mGluR2 as an endocytic receptor to enter cells
a, Outline of the ion channel RNAi screening procedures. Wild-type A549 cells, non-targeting scramble siRNA (siControl)-transfected cells, and siRNA targeting the nucleoprotein (siNP) of H1N1-transfected cells were used as mock, negative, and positive controls, respectively. b, 21 ion channels are involved in the replication of H1N1 virus in A549 cells from the human ion channel siRNA library screen. Indicated siRNAs were individually transfected into A549 cells for 72 h and followed by infection with H1N1 virus at an MOI of 0.01 in three independent experiments. Infectious viral particles in the supernatants were determined at 24 p.i. as TCID50/mL and analyzed by calculating the fold-change of inhibition. The data are means ± s.d. (n = 3 biologically independent experiments).
