Table 1 Activity assays of the cloned candidate DMSP synthesis genes
From: Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microorganisms
Cloned gene | NCBI accession number/MMETSP ID | Candidate DMSP synthesis protein | DMSP or DMSHB production from MTHB in E. coli | DMSP production from DMSHB in E. coli (or R. pomeroyi, Rp) | DMSHB and DMSP production from MTHB in the L. aggregata dsyB−mutant |
|---|---|---|---|---|---|
Control (host with no cloned gene) | – | – | NT | NT/ND (Rp) | ND |
G. sunshinyii YC6258 | WP_044616208.1 | GsDsyGD | 177.42 ± 3.23 | 13.81 ± 0.97 | 471.81 ± 24.89 |
Symploca sp. SIO3E6 | NES17792.1 | DsyGD | 83.83 ± 6.24 | 35.13 ± 1.23 | 147.31 ± 4.27 |
Oscillatoria sp. SIO1A7 | NER39123.1 | DsyGD | 6.69 ± 0.35 | 9.16 ± 0.50 | 93.34 ± 4.27 |
Z. navalis LEGE 11467 | WP_264320056.1 | ZnDsyG | 1.79 ± 0.13 | ND | 1.96 ± 0.13 |
Planctomycetales bacterium | MCA9139260.1 | DsyG-like | ND | ND | ND |
P. umbilicalis | OSX77567.1 | DsyG-like | ND | ND | NT |
Norrisiella sphaerica BC52 | MMETSP0113 | Clade A DSYE | 2.93 ± 0.12 | ND | 0.93 ± 0.02 |
Bigelowiella longifila CCMP242 | MMETSP1359 | Clade A DSYE | 1.54 ± 0.05 | ND | NT |
O. prasinos BCC99000 | MMETSP0933 | Clade B DSYE | 1.13 ± 0.02 | ND | NT |
Tetraselmis striata LANL1001 | MMETSP0803 | Clade B DSYE | 1.58 ± 0.08 | ND | NT |
Pelagococcus subviridis CCMP1429 | MMETSP0882 | Clade C DSYE | 2.06 ± 0.02 | ND | 1.92 ± 0.05 |
C. mesostigmatica CCMP1168 | MMETSP0047 | Clade C DSYE | 1.94 ± 0.04 | ND | NT |
Pavlova sp. CCMP459 | MMETSP1381 | Clade D DSYE | 1.19 ± 0.13 | ND | NT |
Exanthemachrysis gayraliae RCC1523 | MMETSP1464 | Clade D DSYE | 1.43 ± 0.07 | ND | NT |
F. cylindrus CCMP1102 | OEU16654.1 | Clade E DSYE | 0.54 ± 0.03 | ND | 1.48 ± 0.13 |
N. inconspicua | KAG7362955.1 | Clade E DSYE | 1.24 ± 0.03 | ND | 1.35 ± 0.07 |
P. parvum Texoma1 | MMETSP0008 | DsyD-like | NT | ND (Rp) | NT |
A. monilatum CCMP3105 | MMETSP0093 | DsyD-like | NT | ND (Rp) | NT |
