Extended Data Fig. 1: Workflow for CryoFIB/SEM and CryoET.
From: Molecular plasticity of herpesvirus nuclear egress analysed in situ
a, A low magnification SEM image of PrV-ΔUS3-infected porcine epithelial cells. b, A higher magnification image of the box shown in a. c, An oblique SEM view illuminated by the FIB with yellow boxes indicating the area above and below the cell for targeting with the focused ion beam. d, SEM image of thinned cellular section (lamella) from the top and side (inset). Transmission electron microscope (TEM) image is also shown (right), with target area shown in e highlighted by a white box. e, A TEM image shown at 9500x nominal magnification. Details of the cell are visible at this magnification, allowing targeting of regions of interest for higher magnification tomographic data collection (red box). Shown are representative samples of 113 lamella from 3 biological replicates. cyt = cytosol, nuc = nucleus. f, A tomographic slice of the region indicated in e) at 35000x magnification, nominal. ONM, outer nuclear membrane; INM, inner nuclear membrane.
