Extended Data Fig. 2: Quantification of nuclear egress events.
From: Molecular plasticity of herpesvirus nuclear egress analysed in situ
a, Size and curvature distribution of perinuclear vesicles. Shown are inner luminal diameters of individual vesicles (see Methods) with mean values represented by red bars. *The mean value and standard deviation of perinuclear vesicles obtained by expression pUL31/pUL3410 are shown for comparison. Vesicles (and the cargo within) were counted as perinuclear where envelopment was judged to be more than 50% completed (for example Fig. 1c would be considered perinuclear). This is because a significant proportion of vesicles (~150 nm diameter) were only partially contained within the 150–250 nm thick tomograms (that is cut off during FIB milling). b, Quantification of capsid types observed during nuclear egress in porcine epithelial (PrV) and African Green monkey cells (HSV-1). c, Quantification of perinuclear vesicles. Classes were assigned to vesicles based on cargo type. In contrast to vesicle diameter measurements shown in a, vesicles containing procapsids were included in the capsid class, whereas scaffolds were counted as non-capsid cargo (note that there were 6 perinuclear procapsids). Procapsids contain capsid proteins some of which could be involved in the initiation of envelopment and therefore fit better in the ‘capsid’ class. At the same time, all perinuclear procapsids were only partially assembled and consequently the perinuclear vesicles were smaller. d, Diagrams indicating capsid classification. A-capsids lack both nucleic acid and scaffold, B-capsids contain a scaffold, and C-capsids are capsids after completion of DNA packaging. Procapsids (not shown) are C-capsid precursors.
