Extended Data Fig. 6: Selective internalization into goblet cells allows P. aeruginosa to invade lung epithelia. | Nature Microbiology

Extended Data Fig. 6: Selective internalization into goblet cells allows P. aeruginosa to invade lung epithelia.

From: Pseudomonas aeruginosa breaches respiratory epithelia through goblet cell invasion in a microtissue model

Extended Data Fig. 6

a-d. Representative examples of 100× ICC images of P. aeruginosa attaching to and invading lung epithelial cells at 6 h and 12 h p.i. Attached (a), internalized (b) and replicating bacteria (c) are shown. Goblet cell invasion is illustrated in (d) using an additional stain for mucus, and was consistently observed in all three donors tested (e). For all panels: Inoculum = 103. n = 3 independent experiments. For a-d: same donor, for e: different donors. Staining: bacteria: constitutive chromosomal expression of mNeonGreen (yellow) or mCherry (pink), nuclei: DAPI, mucus: Muc5AC, membrane: CellMask, actin: phalloidin-647, tight junctions: ZO-1 Alexa Fluor 555.

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