Extended Data Fig. 9: P. aeruginosa breaching and basolateral invasion of the lower airway epithelium. | Nature Microbiology

Extended Data Fig. 9: P. aeruginosa breaching and basolateral invasion of the lower airway epithelium.

From: Pseudomonas aeruginosa breaches respiratory epithelia through goblet cell invasion in a microtissue model

Extended Data Fig. 9

a, b. Schematic (a) and 100× ICC images (b) of P. aeruginosa invasion of the basolateral side of the lung epithelium after successful establishment of a breaching site. c. Progression of lower airway infection by P. aeruginosa PAO1 wild type recorded by SEM. d, e. Immunocytochemistry images of P. aeruginosa breaching sites seen for all three donors tested (d) and for the PAO1 ΔpscC complemented mutants (e). For all panels: Inoculum = 103. n = 3 independent experiments (same donor [a-c and e] or different donors [d]). ICC staining: bacteria: constitutive chromosomal expression of mNeonGreen, nuclei: DAPI, tight junctions: ZO-1 Alexa Fluor 555.

Back to article page