Extended Data Fig. 10: Rapid colonization of breaching sites is driven by chemotaxis. | Nature Microbiology

Extended Data Fig. 10: Rapid colonization of breaching sites is driven by chemotaxis.

From: Pseudomonas aeruginosa breaches respiratory epithelia through goblet cell invasion in a microtissue model

Extended Data Fig. 10

Breaching site colonization in samples infected with a 1:1 mixture of differentially labeled (pink or yellow) wild type and ΔcheA1/2 mutant strains. a. Wild type + wild type example of co-colonized breach. b. Wild type + ΔcheA1/2 example of co-colonized breach. c. Wild type + ΔcheA1/2 example of a breach solely colonize by wild type. d. ΔcheA1/2 + ΔcheA1/2 example of breaches colonized by only one of the two ΔcheA1/2 strains. For all panels: inoculum = 103, n = 3 independent experiments (same donor). Staining: bacteria: constitutive chromosomal expression of mNeonGreen or mCherry, nuclei: DAPI, actin: phalloidin-647.

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