Extended Data Fig. 4: The enzymatic activity of HgCht2 is important for H. glycines-mediated suppression of rhizobia nodulation and mycorrhizal fungal symbiosis.
a, Nodules on hairy roots expressing HgCht2D129A/E131A or HgCht2 at 14 dpi. Hairy roots expressing GFP are used the control. Bar = 3 mm. b, Number of infection threads in hairy roots expressing HgCht2D129A/E131A and HgCht2 at 3 dpi. Data are shown as means ± SE (n = 5 biologically independent samples). c, qRT-PCR analysis of HgCht2 expression in hairy roots expressing HgCht2D129A/E131A or HgCht2 at 14 dpi. Data are shown as means ± SE (n = 3 independent experiments). d, Nodulation symptoms of soybean roots at 14 dpi upon treatment with HgCht2D129A/E131A or HgCht2. Bar = 3 mm. e, Nodule number of soybean roots at 14 dpi following treatment with HgCht2D129A/E131A or HgCht2. Soybean roots treated with PBS buffer were used as the control. Data are shown as means ± SE (n = 14 biologically independent samples) of one representative experiment. The experiment was repeated three times with similar results. f, Nodulation symptoms of soybean roots at 14 dpi with B. diazoefficiens under the HgCht2 treatment with or without 1516b. Bar = 5 mm. g, Infective thread formation in soybean roots at 3 dpi treated with HgCht2 and 1516b. White arrow, infected thread. Bar = 10 μm. h, Transcript expression levels of the early symbiosis marker genes GmENOD40 and GmNIN at 3 dpi under treatment with HgCht2 and 1516b. Data are shown as means ± SE (n = 3 independent experiments). i, Nodules of soybean roots at 14 dpi when treated with HgCht2 in the presence or absence of 1516b. Bar = 3 mm. j, Mycorrhizal fungal structures in soybean roots are shown at 7 dpi with R. irregularis in the presence of HgCht2 with or without 1516b. Bar = 100 μm. Soybean roots treated with PBS buffer were used as controls. Asterisks in b, c, e, h represent statistically significant differences (Student’s two-sided unpaired t-test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
