Extended Data Fig. 4: Yersinia infection activates AMPK.
From: Yersinia infection induces glucose depletion and AMPK-dependent inhibition of pyroptosis in mice
a, Primary BMDMs were infected with Y. pseudotuberculosis (MOI 40) for the indicated time. Activation of mTORC1 was indicated by p-T389 S6K1 immunoblotting. b, c, Primary BMDMs were stimulated with S. Typhimurium (MOI 100) (b) or LPS (100 ng/ml) (c) for the indicated time. Activation of AMPK was indicated by p-S79 ACC immunoblotting. d, e, Activation of endogenous AMPK in mouse liver (d) and peritoneal macrophages (e) was indicated by p-T172-AMPK and p-S79ACC immunoblotting in response to Y. pseudotuberculosis infection (1 × 108 CFU) for 6 hours. n = 2 mice for each group. The relative levels of p-T172 AMPK and p-S79 ACC normalized to Tubulin levels are indicated below the respective blot. f, Immunohistochemical staining for CD163 as a marker of M2 macrophages in the liver and spleen of 8-week-old mice with or without Y. pseudotuberculosis infection (1 × 108 CFU) for two days. Representative images of n = 3 mice for each group are shown. g, Immunoblotting for CD163 as a marker of M2 macrophages in peritoneal macrophages isolated from 8-week-old mice with or without Y. pseudotuberculosis infection (1 × 108 CFU) for two days. n = 3 mice for each group.
