Extended Data Fig. 1: Yersinia infection results in a decrease in intracellular glucose levels.

a-d, Volcano plot of metabolites (a and b) and pathway enrichment analysis of significantly regulated metabolites (c and d) in primary BMDMs with or without S. Typhimurium (S.t.) infection (MOI 100) or LPS (100 ng/ml) stimulation for 1 hour. The hits were selected based on the fold change of the n = 6 biologically independent samples in each group with cut-offs set at log2(S.t./UT) or log2(LPS/Veh) > 0.5 or < −0.5 and P value < 0.05. Glucose is labeled in red. ATP, ADP, and AMP are labeled in blue. e, Heatmap showing the degree of metabolite level changes in primary BMDMs stimulated with Y. pseudotuberculosis (Y.p., MOI 40), S. Typhimurium (MOI 100) and LPS (100 ng/ml) for 1 hour relative to the untreated control. f-h, Relative abundances of glucose in primary BMDMs stimulated with S. Typhimurium (MOI 100) (f), LPS (100 ng/ml) (g), WT or YopJ-deficient Y. pseudotuberculosis (h) for 1 hour were measured. i, j, Volcano plot of metabolites (i) and pathway enrichment analysis of significantly regulated metabolites (j) in primary lymphocytes with or without Y. pseudotuberculosis (MOI 40) infection for 1 hour. The hits were selected based on the fold change of the n = 6 biologically independent samples in each group with cut-offs set at log2(Y.p./UT) > 0.5 or < −0.5 and P value < 0.05. Glucose is labeled in red. ATP, ADP, and AMP are labeled in blue. k, Relative abundances of glucose in primary lymphocytes infected with Y. pseudotuberculosis for the indicated time were measured. l, Food consumption was monitored for 8-week-old male mice infected with Y. pseudotuberculosis (1 × 10⁸ CFU). Mean ± s.e.m. of n = 6 biologically independent samples (f-h and k) or mice (l) in each group. Unpaired two-tailed Student’s t-test (a-d and f-i). One-way ANOVA post hoc Dunnett’s tests (k). Two-way ANOVA post hoc Bonferroni’s tests (l). UT, untreated. Veh, vehicle. Ctrl, control.

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