Extended Data Fig. 2: Effect of glucose and tryptophan supplementation on production of tryptophan metabolites.
(a) Concentration of tryptophan metabolites in the mGAM medium and culture supernatants of C. sporogenes grown in mGAM medium supplemented with final concentrations of 0.05, 0.2 or 0.4 and 0.6 % glucose. Bars and error lines show mean ± s.d. of three independent biological replicates. (b) Concentration of tryptophan metabolites in the mGAM medium and culture supernatants of P. anaerobius grown in mGAM medium supplemented with final concentrations of 0.05, 0.2 or 0.4 and 0.6 % glucose. Bars and error lines show mean ± s.d. of three independent biological replicates. (c) Absolute concentrations of tryptophan metabolites in the culture supernatants of six infant faecal microbiotas and culture medium control (media control). The six different infant faecal microbial communities are cultured either in YCFA medium or YCFA supplemented with 0.05 or 0.1 or 0.2 % of free tryptophan. (d) 16S rRNA gene sequencing profile of the six infant faecal microbiota compositions.
