Extended Data Fig. 8: SARS-CoV-2 WT challenge infection of OTS-228 immunized Syrian hamsters.
a, Experimental setup. Syrian hamsters (Mesocricetus auratus, male, 12 weeks old) were OTS-228 vaccinated and subsequently challenged by SARS-CoV-2 WT. Overview created with BioRender.com. b, Survival post-challenge infection. c, Relative body weight in percent. d, Virus genome copy numbers in nasal washing (until dpc5: n=8 OTS-228 and mock group, n=3 OTS-228 contact group; from dpc 6 on: n=3 OTS-228 and contacts and n=4 mock group) and e, organ samples of 5 dpc (n=5 OTS-228 group, n=4 mock group). f, Genome loads of organ samples taken 14 dpc (¾ mock vaccinated animal sampled on 7dpc) g, Serum samples of 5 and 14 dpc were analyzed by SARS-CoV-2 RBD-ELISA. h, Serum samples that reacted positively in the ELISA, were analyzed in addition by live virus neutralization assay (capacity to neutralize 100 TCID50) against ancestral (B.1) SARS-CoV-2 as well as against Omicron BA.2 and BA.5 variants. Statistical significance was assessed by two-sided, unpaired, nonparametric multiple t-test with Mann-Whitney test (compared ranks) (panel c-e). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. No asterisk indicates no statistical significance.
