Extended Data Fig. 9: Omicron BA.2 challenge of OTS-228 vaccinated Syrian hamsters. | Nature Microbiology

Extended Data Fig. 9: Omicron BA.2 challenge of OTS-228 vaccinated Syrian hamsters.

From: A safe, effective and adaptable live-attenuated SARS-CoV-2 vaccine to reduce disease and transmission using one-to-stop genome modifications

Extended Data Fig. 9

a, Experimental setup. Syrian hamsters (Mesocricetus auratus, male, 12 weeks old) were OTS-228 vaccinated and subsequently challenged by SARS-CoV-2 BA.2. Data obtained from n = 8 OTS-228 inoculated hamster from one experiment, with n=3 naïve direct contact hamsters. Overview created with BioRender.com. b, Survival post challenge infection. c, Relative body weight in percent. Shown are the mean with standard error. d, Virus genome copy numbers in nasal washing (until dpc5 n=8 and n=3 (contacts), from dpc6 on n=3 for both groups) and e, organ samples of 5 dpc (n=5 OTS-228) and f, 14 dpc (n=3 OTS-228 and contacts). g, Serum samples of 5 and 14 dpc were analyzed by SARS-CoV-2RBD-ELISA. h, Serum samples which reacted positively in the ELISA, were analyzed in addition by live virus neutralization assay (capacity to neutralize 100 TCID50) against ancestral (B.1) as well as against Omicron BA.2 variant.

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