Extended Data Fig. 3: Comparison of the inflammatory response induced by M. tuberculosis complex L6 (a-b) and L4 (c-d) strains in human monocyte derived macrophages (MDMs) based on their self reported ancestry colocalizing with L6 at 24 (a-c) and 96 (b-d) hours post-infection.
From: Differential rates of Mycobacterium tuberculosis transmission associate with host–pathogen sympatry
Six human inflammatory cytokines-chemokines were screened using LEGENDplex. The stacked bars represent the mean production of IL-1ß, TNF-α, MCP-1, IL-6, IL-8, and IL-18. Each bar represents three donors colocalizing with strains of Lineage 6 (Yes [Nigerian, Cameroonian, Ghanaian]) and no colocalizing with strains of Lineage 6 (No [German donors]). PBS, Macrophage Infection Media (MIM), and supernatants from not infected MDMs were used as controls. The MIM values were subtracted from the not infected and infected MDMs. Mean, standard error of the mean, and significant statistical results (*P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001) are shown. Statistical results were calculated based on Two-way ANOVA multiple comparison with Bonferroni correction. Data were obtained from six independent infection experiments (three for each donor group). L6, Lineage 6; L4, Lineage 4; hpi, hours post-infection.
