Extended Data Fig. 7: Vascular protection is conferred by a conserved functional domain in the N-terminal part of ANGPTL4.
From: Angiopoietin-like 4 protects against endothelial dysfunction during bacterial sepsis
a, Structure prediction of Peptide 4 was provided using PSIPRED Workbench (http://bioinf.cs.ucl.ac.uk/psipred/). b, Sequence homology between human and mouse ANGPTL4; a.a. 66-80 encompassing the functional motif are highlighted in yellow. c, HDMEC either uninfected or infected with N. meningitidis 2C4.3 for 30 min were treated with 1 μg.mL−1 of recombinant human or mouse ANGPTL4 or vehicle for 2 h and 30 min. Cells were fixed and stained for cellular VE-cadherin, Bacteria and DAPI and analysed by fluorescent microscopy (scale bars, 50 μm). Quantification of VE-cadherin enrichment at intercellular junctions were performed with ImageJ software (n = 5 to 6 fields from one representative experiment performed in duplicate). Error bars show mean ± s.e.m.; One-way ANOVA Tukey’s multiple comparison test ****P < 0.0001; NS, not significant (NS1 = 0.3515, NS2 = 0.8094).
