Extended Data Fig. 1: Brain microvascular endothelial cells have intrinsic capacities to regulate inflammation in response to meningococcal infection.
From: Angiopoietin-like 4 protects against endothelial dysfunction during bacterial sepsis
a, A schematic representation of the protocols used in vitro to conduct the RNA sequencing analysis on primary human dermal (HDMEC) or brain (HBMEC) microvascular endothelial cells, created with BioRender.com. Cells were either uninfected or infected with N. meningitidis 2C4.3 for 3 h, mRNA were extracted and a global transcriptional analysis was performed to identify the differentially expressed genes (DEGs, see method, n = 3 samples per condition). The number of upregulated (red) and downregulated genes (blue) are indicated. Bottom, Venn diagram representing the DEG repartition in both cell types. b, Percentage of meningococcal read abundances in the sequenced samples showing similar levels of infection in both cell types. Error bars show mean ± s.e.m. c, Log2Fold changes of the 101 genes commonly regulated in both HDMEC and HBMEC upon infection (see details in Supplementary Table 3). d, Radar plots showing the production of inflammatory factors in supernatants of uninfected and infected HDMEC or HBMEC. Supernatants were collected after 3 h of infection and factors were measured with multiplex electrochemiluminescent immunoassays. Data are presented as relative fold changes from one representative experiment performed in quadruplicate with at least two independent biological replicates using two different donors.
