Extended Data Fig. 2: ANGPTL4 produced by brain microvascular endothelial cells preserves endothelial integrity in response to meningococcal infection.
From: Angiopoietin-like 4 protects against endothelial dysfunction during bacterial sepsis
a, hCMEC/D3 were either uninfected or infected with N. meningitidis 2C4.3, cell supernatants were collected after 3 h of infection and the production of ANGPTL4 was quantified with electrochemiluminescent immunoassay. Error bars show mean ± s.e.m from one experiment performed in triplicate. b, hCMEC/D3 were either uninfected or infected with N. meningitidis 2C4.3 for 3- to 9-h. Cells were fixed and stained for cellular VE-cadherin, bacteria, and DAPI and analysed by fluorescent microscopy. The images are representative of VE-cadherin staining of two independent experiments performed in triplicate (scale bars, 50 μm). c, Extended data of the images shown in Fig. 1f (scale bars, 50 μm).
