Extended Data Fig. 4: Experimental autoimmune encephalomyelitis disease course in germ-free, SM01-, SM13- and SM14-colonized mice.
From: Gut microbial factors predict disease severity in a mouse model of multiple sclerosis
a, Left, maximum achieved EAE score per individual (Max) within FR-fed SM13-colonized mice, FF-fed SM13-colonized mice, FR-fed SM14-colonized mice and FF-fed SM14-colonized mice. Wilcoxon rank-sum test with p-value adjustment using the Benjamini-Hochberg method. No statistically significant group differences were observed. Right, percentage of variance explained by diet (FR vs. FF) and SM combination (SM13 vs. SM14) as determined by eta-squared calculation when comparing the maximum achieved EAE score during the 30 d disease observation period between FR- and FF-fed SM14- and SM13-colonized mice. b–d, Germ-free (GF) C57BL/6N mice were either monocolonized with A. muciniphila (SM01) or remained GF and were fed either FR or FF diet. SM01 mice were only fed the FR diet. EAE was induced in GF mice 20 d after diet switch. EAE was induced in SM01-colonized mice 25 d after initial colonization. Disease course in all groups was observed for 30 days after EAE induction. b, EAE disease scores as a function of time (days after EAE induction) for FR-fed GF mice, FF-fed GF mice and FR-fed SM01-colonized mice. Dots represent daily group means. Dashed lines represent SD. c, Sankey diagram of key event occurrence (in % of all mice within one group) during EAE. d, AUC analysis of the disease course depicted in panel b. Each mouse is depicted by a separate dot. One-way ANOVA followed by Tukey’s post-hoc test. No statistically significant group differences were observed. Mouse numbers are indicated on the respective panels and treated as biological replicates. Boxplots (a, d) show median, quartiles, and 1.5 × interquartile range.
