Extended Data Fig. 8: Microbiota-associated predictors for experimental autoimmune encephalomyelitis development.
From: Gut microbial factors predict disease severity in a mouse model of multiple sclerosis
a, Strain relative abundances of SM12-, SM13- and SM14-colonized mice, irrespective of diet, before induction of EAE. b, Linear mixed model regression for predicted maximum score during EAE (Max) and mean score during relapse phase (RelM) with presence of the strain as an independent variable and colonization as a random intercept effect. c, Concentrations of secretory IgA (sIgA) in faecal samples samples collected 30 d after EAE induction, normalized to faecal weight. Mice grouped by individual EAE phenotype (Fig. 5e). Due to lack of available material, sIgA could not be measured for some mice. Cluster 1, strong EAE symptoms; Cluster 2, mild EAE symptoms. Wilcoxon rank-sum test. d, Concentrations of faecal sIgA, normalized to faecal weight. Mice grouped by colonization–diet combination and EAE induction status (taken after 30 d of EAE for EAE-induced mice). Kruskal-Wallis test followed by Wilcoxon rank-sum test. Statistical differences are indicated by compact letter display: two groups sharing an assigned letter, non-significant; two groups not sharing an assigned letter, p < 0.05. Right panel, variance of sIgA concentrations explained by either SM or diet. nd, not done. e, Correlation between disease susceptibility among EAE-induced mice harbouring a certain SM with mean faecal sIgA levels in non-EAE-induced mice harbouring the same SM by linear regression. Each dot represents one SM combination. f, IgA coating index (ICI) of each strain dependent on SM. One-way ANOVA. See Methods for details on ICI calculation. ICIs could not be calculated for M. formatexigens as the relative abundance was below the limit of detection for at least one of the sorted fractions across all samples (also applies for panels g and h). na, not applicable; ns, non-significant. g, Variance in IgA coating index (ICI) explained by background microbiota (SM12, SM13 and SM14; FR only) by eta-squared (η2) calculation. Variances could not be calculated for A. muciniphila, as it was only present in one microbiota combination. h, Classification of SM14-constituent strains as IgA high-coated, intermediate, or low-coated, depending on microbiota composition. Boxplots (a, c, d, f) show median, quartiles, and 1.5 × interquartile range.
