Fig. 4: Microbial mucin degradation is disconnected from EAE disease course.
From: Gut microbial factors predict disease severity in a mouse model of multiple sclerosis
a, Relative abundances of strains that provided statistically significant differences between FR-fed SM14-colonized mice and FR-fed SM13-colonized mice on the day of EAE induction, as determined using one-way ANOVA followed by multiple comparisons with P-value adjustment using the Benjamini–Hochberg method. Only biologically meaningful multiple comparisons made (same SM, different diet; different SM, same diet). b, Constituent strains of the SM communities. Strain abbreviations as in Fig. 2a. c, EAE disease scores as a function of time. Daily EAE scores were compared using Wilcoxon rank-sum tests with P-value adjustment using the Benjamini–Hochberg method. Blue asterisks represent comparison with FR-fed SM13-colonized mice, while yellow asterisks represent comparison with FR-fed SM14-colonized mice. *P < 0.05. d, Sankey diagram of key event occurrence (in % of all mice within one group) during EAE. e, Left: AUC analysis of the disease course depicted in c. Each mouse is depicted by a separate dot. Middle: maximum EAE score per mouse (Max). Right: mean EAE score during relapse phase (RelM, day 26 to day 30 after EAE induction). Analysed using one-way ANOVA followed by Tukey’s post-hoc test for groupwise comparisons (AUC and RelM) or Wilcoxon rank-sum tests with P-value adjustment using the Benjamini–Hochberg method (Max). Blue text indicates comparison with FR-fed SM13-colonized mice, while yellow text indicates comparison with FR-fed SM14-colonized mice. NS, not significant. Mouse numbers are indicated on the respective panels and treated as biological replicates. FR-fed SM14 and SM13 data are from three independent experiments; FF-fed SM14 data are from two independent experiments; all other groups are from one experiment. Boxplots (a,e) show median, quartiles and 1.5× IQR. All statistical tests were two-sided.
