Extended Data Fig. 6: Bismuth disrupts membrane potential and promotes intracellular antibiotic accumulation in P. aeruginosa.
a, Detection of membrane potential in P. aeruginosa. Red/green ratios were calculated using population mean fluorescence intensities for P. aeruginosa incubated with 15 μM DIOC2(3) for 30 min in the presence of BSS or 20 μM CCCP. b, The time-based intracellular bismuth level of PAO1 under the treatment of 32 μM BSS. c–e, Intracellular BSS dose-dependent accumulation of azithromycin, chloramphenicol and ofloxacin. PAβN was used as a positive control. For Fig. a–e, the data are presented as mean ± SEM of three biological replicates. P values were determined using two-sided unpaired t-test for Fig. a, c–e.
