Extended Data Fig. 3: Effect of chaperones, oxygen, and the stringent response on temperature upshift responses.
From: Metabolic rearrangement enables adaptation of microbial growth rate to temperature shifts
a) Single-cell growth rates of E. coli MG1655 on rich medium (LB) undergoing a temperature upshift from 25 °C to 37 °C in aerobic (blue, n=773 cells) or anaerobic (orange, n=319 cells) conditions. Data are the mean±1 SEM (shaded region) at each time point. b) Normalized growth rate versus thermal time for each trajectory in (a). c) Single-cell growth rates of ΔdnaK (red, n=433 cells) and its parent BW25113 (blue, n=1011 cells) on rich medium (LB) undergoing a temperature upshift from 37 °C to 42 °C. Data are the mean±1 SEM (shaded region) at each time point. d) Single-cell growth rates of ΔdnaK (red, n=318 cells) and its parent BW25113 (blue, n=734 cells) on rich medium (LB) undergoing a temperature upshift from 27 °C to 37 °C. Data are the mean±1 SEM (shaded region) at each time point. e) Normalized growth rate versus thermal time for each trajectory in (d). f) Single-cell growth rates of a ppGppnull strain (ΔrelA ΔspoT) (purple, n=648 cells) and its parent MG1655 (blue, n=792 cells) on rich medium (LB) undergoing a temperature upshift from 27 °C to 37 °C. Data are the mean±1 SEM (shaded region) at each time point. g) Normalized growth rate versus thermal time for each trajectory in (f). h) Single-cell growth rates of ppGppnull (purple, n=47 cells) and its parent MG1655 (blue, n=474 cells) on rich medium (LB) undergoing a temperature upshift from 27 °C to 42 °C. Data are the mean±1 SEM (shaded region) at each time point.
