Extended Data Fig. 2: Metal levels in Zn sufficient and Zn deficient mice during A. baumannii pneumonia at 24 hpi.
From: Dietary zinc deficiency promotes Acinetobacter baumannii lung infection via IL-13 in mice
(A–D) Total iron (Fe) and copper (Cu) levels in the lungs and serum (Ctrl Zn mock, n = 5; Low Zn mock, n = 5; Ctrl Zn infected, n = 5; Low Zn infected, n = 4; p by one-way ANOVA with Sidak’s multiple comparisons; mice were male 9-week old C57BL/6J). (E, F) WT and ∆znuC A. baumannii 17978 growth in lysogeny broth (LB) with and without additional Zn (1.25 mM ZnCl2; n = 3 per group; p by two-sided Student’s t-test; data are representative of at least two independent experiments). (G) Survival of male 9-week old C57BL/6J mice fed control Zn or low Zn diet and infected with a 1:1 mix of WT and ∆znuC A. baumannii 17978 (Ctrl Zn, n = 14, Low Zn, n = 12; p by two-sided Mantel-Cox test). Graphs show mean ± SEM.
