Extended Data Fig. 3: Lung barrier measures, myocardial necrosis, and flow cytometry gating.
From: Dietary zinc deficiency promotes Acinetobacter baumannii lung infection via IL-13 in mice
(A) Wet lung weight at 24 hpi of 9-week old male C57BL/6J mice (Ctrl Zn mock, n = 5; Low Zn mock, n = 5; Ctrl Zn infected, n = 5; Low Zn infected, n = 4; p by one-way ANOVA with Sidak’s multiple comparisons). (B, C) Serum albumin in bronchial alveolar lavage fluid (BALF) at 16 hpi (Ctrl Zn mock, n = 2, Low Zn mock, n = 2; Ctrl Zn infected, n = 4; Low Zn infected, n = 5) and 24 hpi (Ctrl Zn mock, n = 2; Low Zn mock, n = 2; Ctrl Zn infected, n = 3; Low Zn infected, n = 4). p by one-way ANOVA with Sidak’s multiple comparisons, mice were 9-week old male C57BL/6J. (D) Representative images of myocardial necrosis clusters in hearts of 12-week old male C57BL/6J mice. (E) Myocardial necrosis scoring, n = 5, p by two-sided Mann-Whitney test. (F) Gating strategy using a representative male C57BL/6J mouse fed low Zn diet and infected with A. baumannii 17978. Abbreviations: FSC-H, forward scatter height; eos., eosinophils; Alv. Mac., alveolar macrophages; DC, dendritic cell; FMO, fluorescence minus one. Graphs show mean ± SEM for parametric data and median for non-parametric data.
