Extended Data Fig. 4: Bacterial burdens, IL-13 abundance, and quantification of viable cells in investigating IL-13 production.
From: Dietary zinc deficiency promotes Acinetobacter baumannii lung infection via IL-13 in mice
(A) A. baumannii 17978 CFU in the lungs of 9-week old male C57BL/6J mice shown in Fig. 4a,b (Ctrl Zn, n = 5; Low Zn, n = 4). (B) IL-13 abundance in the lung samples shown in Fig. 4 measured by Luminex (Ctrl Zn mock, n = 5; Low Zn mock, n = 5; Ctrl Zn infected, n = 5; Low Zn infected, n = 4). p by Kruskal-Wallis with Dunn’s multiple comparisons). (C) Lungs of 9-week old male C57BL/6J mice fed ctrl Zn or low Zn diet were digested and restimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin for 6 h and viable cells were enumerated pre- and post-restimulation by trypan blue exclusion with a hemocytometer (Ctrl Zn mock, n = 3; Low Zn mock, n = 3; Ctrl Zn infected, n = 7; Low Zn infected, n = 7; p by two-sided ratio paired t test with Holm-Sidak’s multiple comparisons). (D) IL-13 abundance in spleen samples from 12-week old male (solid symbols) and female (divided symbols) C57BL/6J mice was measured by ELISA (6 hpi, Ctrl Zn, n = 9, Low Zn, n = 10; 16 hpi, n = 9; 24 hpi, n = 10; p by two-sided Mann-Whitney at each timepoint). Panels A, B, and D show median; Panel C shows mean ± SEM.
