Extended Data Fig. 2: Relative and absolute abundance profiles of individual faecal samples incubated with entacapone.
From: The Parkinson’s disease drug entacapone disrupts gut microbiome homeostasis via iron sequestration
a. Total microbial cell loads in incubations of samples from three different donors (compared to samples used in main text, for example, Fig. 1) with entacapone (ENT-Hi) and controls (No drug - DMSO only), at the start of the incubation (time=0 h, t0) and after 6 and 24 h in sM9 medium. Total cell loads were assessed by flow cytometry. Indicated P values are from an unpaired two-sided t-test with “No drug” as a reference group. Data points represent the mean value +/- SEM from three incubation vials per condition. b, c. Relative (b) and absolute (c) genus abundance profiles of faecal microbial communities (Donor 1: D1, Donor 2: D2 and Donor 3: D3) incubated with ENT-Hi and assessed by 16S rRNA gene amplicon sequencing. The community composition at 0 h (inoculum) is also shown. All genera present at a relative abundance below 0.025 or absolute abundance below 5x106 cells.ml-1 were assigned to the category “Other”. Each bar represents the mean from triplicate incubations.
