Extended Data Fig. 3: Pump probe microscopy combined with widefield fluorescence imaging for correlative chemical and fluorescence in situ hybridization imaging.
From: The Parkinson’s disease drug entacapone disrupts gut microbiome homeostasis via iron sequestration
a. Experimental setup. Left dashed box: laser point scanning mode for SRS/PT. DL: delay line; HWP: half waveplate; PBS: polarized beam splitter; AOM: acousto-optic modulator; DM: dichroic mirror; SM: scanning unit; FM: flip mirror; OBJ: objective; COND: condenser; SP: short pass filter; PD: photodiode; RA: resonant amplifier; Ex: excitation filter; Em: emission filter; TL: tube lens. b. Stimulated Raman scattering energy diagram. \({\omega }_{p}\): pump beam frequency; \({\omega }_{S}\): Stokes beam frequency; \(\hslash\): Planck’s constant; \(\varOmega\): energy of the vibrational mode; v: vibrational level. c. Picosecond stimulated Raman scattering by spectral focusing.
