Extended Data Fig. 5: Effect of trypsin treatment on the infectivity of coronavirus pseudotypes.
From: Receptor-binding proteins from animal viruses are broadly compatible with human cell entry factors
A. Viral suspensions were treated with trypsin as indicated, washed and used to inoculate each of the 51 cell lines. Yellow boxes show host information. Names of cell lines are indicated at the top, and cells are organized by tissue of origin. The heat map shows relative pseudotype infectivity, calculated as log2(R + 1), where R is the multiplicity of infection (MOI) scaled as a percentage of the maximum MOI across all cell lines for each pseudotype. Blue bars on the right indicate the number of cell lines in which infection was detected. B. Mock- or trypsin-treated coronavirus pseudotypes were analyzed by western blot. The spike was detected using an anti-His-Tag antibody. VSV-M was used as a loading control. Spike cleavage by trypsin was detected in most cases. Western blot analysis was performed once.
