Extended Data Fig. 6: Effect of neuraminidase (sialidase) and heparinase III treatment on pseudotype infectivity in SNB-19 cells.
From: Receptor-binding proteins from animal viruses are broadly compatible with human cell entry factors
A. For each pseudotype, the log2(fold change) in the GFP signal in cells pre-treated with neuraminidase (left) or heparinase III (right) relative to untreated controls is shown. Red and blue bars show cases in which treatment increased or decreased infectivity, respectively. All assays were performed twice. NA: not assayed. B. Verification of the neuraminidase and Heparinase III activities. Top: reduction in the infectivity of influenza A virus (IAV) PR8–GFP following pretreatment of cells with neuraminidase. Bottom: immunofluorescence validation of heparan sulfate cleavage by Heparinase III using an antibody specific to digested heparan sulfates (diHS). Scale bars: 100 µm. Representative images of one out of two independent experiments are shown.
