Extended Data Fig. 7: Heat, DNase, RNase, or proteinase treatment of the cell lysate did not reduce the 3D biofilm induction capacity of cell lysate.
From: Bacteria use exogenous peptidoglycan as a danger signal to trigger biofilm formation
V. cholerae WT cells in microfluidic flow chambers were exposed to lysate of V. cholerae WT cells (obtained by sonication, 1010 lysed cells mL−1 in LB medium) which was subjected to different treatments: heat (80 °C for 20 min), DNase I (1 U mL−1 at 37 °C for 30 min), RNase A (1 µg mL−1 at 37 °C for 30 min), or proteinase K (20 µg mL−1 at 37 °C for 60 min). As control conditions, LB medium that was subjected to the same treatments was flushed into flow chambers seeded with V. cholerae WT cells. The heat or enzyme treatments did not diminish the biofilm inducing capability of the lysate. Bars are mean values of n independent biological replicates, where n is as follows for the different conditions (bar1 corresponds to the left-most bar in the graph and bar9 corresponds to the right-most bar): nbar1 = 3, nbar2 = 6, nbar3 = 3, nbar4 = 3, nbar5 = 6, nbar6 = 7, nbar7 = 3, nbar8 = 3, nbar9 = 7. Circles indicate individual measurements, and error bars indicate the standard deviation. Statistical significances were calculated using a one-way ANOVA with Bonferroni’s correction, with the following results: ns = not significant, where untreated lysate vs. heat-treated lysate yielded p = 0.483, untreated lysate vs. DNase-treated lysate yielded p = 0.115, untreated lysate vs. RNase-treated lysate yielded p = 0.142, untreated lysate vs. proteinase K-treated lysate yielded p = 0.0617.
