Extended Data Fig. 7: Antibody Pressure Drives Filament Assembly in Calu3 cells and eggs. Supplement to Fig. 4.
From: Influenza A virus rapidly adapts particle shape to environmental pressures
a. Pairs of plots showing viral yields (left) and shapes (right), measured by flow virometry of supernatants from MDCK cells, 24 hours post-PR8 infection at MOI 0.6, treated with antibodies at a range of indicated concentrations. Black: antibody present throughout infection. Blue: antibody present until 4 h.p.i. to test entry effects. Red: antibody added at 4 h.p.i. to test assembly effects. All antibodies are mouse IgG except Fi6, which is human IgG. b. Shape of virions measured by flow virometry of allantoic fluid from embryonated chicken eggs, 24 hours post-Cal0709 infection at 106 input virions, either untreated or treated with 150 pmol T2-5D antibody at 3 h.p.i. (estimated 3nM final concentration). c. As in a, except with a single concentration of an antibody targeting a cellular transmembrane protein. All: Plotted are mean and S.E.M. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-tailed unpaired t-test relative to an untreated control for the highest antibody concentration. Gray shading indicates yields too low to reliably measure shape. Data plotted derive from 3 biological replicates.
