Fig. 2: Infection efficiency predicts virion shape.
From: Influenza A virus rapidly adapts particle shape to environmental pressures
a, Virion shapes determined by flow virometry at 50 h.p.i. for low MOI (0.006) infections, coloured by cell line. IAV strains are indicated by symbols. n.d., no data. b, The data plotted in a but coloured by viral strain. Cell lines are indicated by symbols. c, Virion shapes for the samples in a, plotted against the infectivity (virions per IU) for the given virus–cell combination. d, Virion shapes for the samples in a, plotted against virion yield produced in the infection, determined by flow virometry. e, The four indicated virus strains were used to infect the four indicated cell lines at a range of MOI (0.016–2). Plotted is the average change in shape distribution for a 10-fold increase in MOI, calculated from log-linear regression analysis, derived from 15–48 data points. Error bars represent the 95% confidence intervals of the regression. f, RT–qPCR for viral genomes (NP segment) were performed on cells at 0, 4, 20 and 21 h.p.i. Plotted is the genome replication versus shape of virions produced by these infections, where average genome replication was calculated as the ratio of genomes at 20.5 h.p.i. (average of values at 20 and 21 h.p.i.) to those at t0. g, Efficiency versus shape plots at low MOI. For all panels, plotted data are mean and s.e.m. of three biological replicates. Spread was prevented in all shown infections. c,d,f,g, r and P values shown are from two-tailed Spearman’s correlation analyses. n.s., not significant, P > 0.05; *P < 0.05 and ****P < 0.0001 by Spearman’s correlation.
