Fig. 4: Antibody pressure drives filament assembly.
From: Influenza A virus rapidly adapts particle shape to environmental pressures
a, Plots of virion efficiency (virions produced/virions input) versus shape, measured by flow virometry of supernatants from MDCK cells at 24 h post-PR8 infection at MOI 0.6, treated with antibodies at a range of concentrations targeting the labelled antigenic site (see Extended Data Figs. 6–8 for individual inhibitor titration curves). Blue: antibody present until 4 h.p.i. to test entry effects. Red: antibody added at 4 h.p.i. to test assembly effects. Plotted are mean and s.e.m. b, As in a, but for PR8 virus in Calu3 cells. c, As in a, but for the H3N2 virus XUdorn in Calu3 cells. Trypsin was not included in infections, which fully prevents spread in a and mostly, but not completely in b and c (Extended Data Fig. 2). a–c, Data plotted are from three biological replicates.
