Fig. 5: Shape dynamics are fast.
From: Influenza A virus rapidly adapts particle shape to environmental pressures
a, MDCK cells infected with PR8 at MOI 0.6 for 20 h were washed into media containing varying concentrations of MEDI8852 antibody. Virion shapes at 1, 2 or 4 h following antibody treatment. b, MDCK cells infected with PR8 at MOI 0.6 were treated with MEDI8852 antibody at 21 h.p.i. Two hours after treatment, cells were fixed and permeabilized. MEDI8852 was stained with an AF647-labelled secondary antibody and nuclei were stained with Dapi (grey). c, As in b, but at MOI 0.3 with directly conjugated AF488-Fi6 IgG treatment at 22 h.p.i. Panels b and c are representative images of antibody internalization that has been observed in four biologically independent experiments. d, MDCK cells infected with PR8 at MOI 0.3 for 20 h were washed into media of varying osmotic strengths. Plotted are virion shapes at 30 min post media change. P values: 0.4×, 8.59 × 10−5; 0.6×, 7.60 × 10−4; 0.8×, 0.0138; +50, 1.24 × 10−3; +100, 9.60 × 10−4; and +150, 1.12 × 10−3. e, As in d, but just for 0.4× media. In addition, samples were alternated between treatment and untreated every 30 min. Plotted are virion shapes for each 30-min interval. P values from left to right: 0.0109, 0.468, 5.87 × 10−3, 0.402 and 0.0258. a,d,e, Plotted are mean and s.e.m. of three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 using unpaired t-test relative to control.
