Extended Data Fig. 3: Supplement to Fig. 2.
From: Influenza A virus rapidly adapts particle shape to environmental pressures
a-d. Virion yields (left) and shape (right), determined by flow virometry of supernatants for the cell line indicated by color infected with a. PR8, b. XUdorn, c. HK68, and d. Cal0709 at high (solid line) and low (dashed line) MOI. Ammonium chloride was added at 4 h.p.i. to prevent spread in each case. e. Viral genomes (NP segment) within MDCK cells infected with PR8 (black), XUdorn (red), or Cal0709 (green) at high MOI (6, solid line) or low MOI (0.006, dashed line) measured by RT-qPCR at 0, 4, 20, and 21 h.p.i. f. As in e, except in Calu3 cells. g. Plotted is the shape of virions produced by infections vs. the ratio of genomes at 4 h.p.i. to 0 h.p.i. (t4/t0), representing early amplification of viral genomes. Also shown are two-tailed Spearman’s correlation r and p values. h. As in g, except plotting the ratio of genomes at 21 h.p.i. to 20 h.p.i (t21/t20), representing late changes in viral genomes. i. Efficiency vs. shape plots for the t50 time points at high MOI, along with two-tailed Spearman’s correlation r and p values. All panels: Plotted are mean and S.E.M. a-i. Data plotted derive from 3 biological replicates.
