Fig. 1: Engineering growth of P. putida on C6-PA monomers.
From: Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida
a, Conceptual overview of PA bio-upcycling through hydrolysis and microbial catalysis, illustrating the chemical structures of monomers and exemplary oligomers derived from different PA substrates. b, Growth of P. putida KT2440-AA and HMDA-1 mutant on diamines. c, ALE of PP_2884∆3 on Ahx as sole carbon source. Shown are the growth profiles of five subsequent cultures. Time points of re-inoculation are indicated. d, Growth of mutants resulting from ALE on Ahx as sole carbon source. e, Target mutations (red asterisks) for reverse engineering yielded by genome sequencing of strain Ahx-194. f–h, Comparative analysis of the growth of the ALE and reverse-engineered strains on HMDA, Ahx and ε-caprolactam, respectively. Strains were cultivated in Growth Profiler in 96-well microtitre plates with MSM containing 15 mM of HMDA, Ahx or ε-caprolactam as sole carbon source. Mean ± s.d. (n = 3 replicates).
