Extended Data Fig. 1: ZIKV infection is inhibited in ARF4 deleted HeLa cells.
From: ARF4-mediated intracellular transport as a broad-spectrum antiviral target
a) CL from WT and ARF4−/− HeLa cells were collected to verify the deletion efficiency by WB with the anti-ARF4 antibody. GAPDH was used as loading control. b) ARF4−/− and WT HeLa cells were challenged with ZIKV at an MOI of 0.1. Culture medium was collected daily until cytopathic effects were observed in WT cells which appeared at 3 dpi. Viral titres were determined by plaque assay on Vero cells and expressed as PFU/ml. Results are shown as means ± SD from three independent experiments. The p-value were determined versus WT by two-way ANOVA with multiple comparisons. The p-value was labelled accordingly hereafter if p < 0.05.
